7 steps for IVF, have you done it right?
IVF operation has high requirements of equipment accuracy, laboratory environment, and doctor’s experience. Therefore, if you want to get conceive, you must follow the doctor's treatment strategy, rather than searing on the web randomly. The development and application of IVF technology have made many people re-recognize this high-tech assisted fertility technology, at the same time they hope it can help them pregnant. More and more older-age families have more fertility difficulties, so they need the help of IVF urgently.
Undoubtedly, the process of IVF and natural pregnancy are definitely different. The whole process of IVF is more complicated. Thus, I will analyze the seven major sections of the IVF process in detail.
Controlled Hype Promoting
Due to women have different natural menstrual cycles, it is not easy to arrange egg retrieval time. Moreover, in the natural cycle, only one dominant follicle develops. After fertilization, only one embryo can be matured, but the pregnancy rate of transferring one embryo is very low.
Therefore, it is necessary to use controlled ovulation to enhance and improve ovarian function, obtain more healthy eggs, and culture multiple embryones to transfer.
It usually takes 8-15 days to stimulate ovulation. It is necessary to strictly follow the doctor's instructions, inject relevant medicines or check up on time, and actively inform the doctor if there is any history of medicine allergy.
During the period of ovulation stimulation, the diet needs to be diversified to ensure adequate nutrition and protein intake; make sure sufficient sleep and avoid overwork; do not do more exercise, fast bending, smoke or drink, and keep away from all kinds of radiation.
It takes about 2-4 days to monitor follicles in clinics by ultrasound monitoring. Through monitoring, the doctors can evaluate the effect of ovarian stimulation, determine the time of egg retrieval, and check the E2 value (estrogen) with a blood draw to adjust the medicine dosage.
When the follicle size is confirmed to reach the requirement, patients can inject human chorionic gonadotropin (HCG) (that is, inject at night) to promote the maturation of the follicles.
34-36 hours after the injection of human chorionic gonadotropin (HCG), doctors will retrieval eggs under the guidance of vaginal ultrasound in local anesthesia. Pass the egg retrieval needle through the vaginal fornix and reach the ovary to suck eggs. Under the microscope, immediately move the egg to a culture dish with an embryo nutrient solution and place it in an incubator for culture.
On the day of egg retrieval, the woman should follow the doctor’s instructions to keep away from salt according to the method of anesthesia, and take antibiotics if necessary; pay attention to personal hygiene, take appropriate rest, and maintain a good mood. 12 hours after egg retrieval, patients can eat properly, better avoid strenuous activities, and don’t have a sex life and take medicines as prescribed by the doctor.
Take the sperm
Sperm retrieval and egg retrieval are performed on the same day. The man needs to excrete once before the woman injects HCG 1-2 days. On the day of the egg retrieval, the man takes the sperm according to the following requirements: before taking the sperm, wash his hands, use masturbation, and save the semen. Do not touch the rim or inside of the cup when storing it. The semen taken out is processed by the upstream method or the Percoll density gradient centrifugation method.
In vitro fertilization
After the eggs are retrieved, the laboratory staff will put the processed sperm and eggs in the same petri dish. After a period of co-cultivation, they can observe the fertilization under a microscope. If the sperm quality is too poor to be fertilized naturally, it must be fertilized by microinjection (ICSI).
Fertilized eggs cultured in vitro for 48-72 hours, it can embryos develop to the 8-16 cell level. Then, according to the patient's age, pregnancy history, and embryo quality, determine the number of the embryo to be transferred and the leftover embryos can be frozen and stored.
Embryo transfer generally does not need anesthesia. Patients should relax and take it easy during transplantation. On the day of transplantation, don’t eat foods such as milk and sweets for breakfast and should drink water appropriately. Moreover, patients should wash the vulva and hold the urine moderately before surgery.
Do not have sex after transplantation, avoid lying down for a long time to prevent thrombosis. Also, patients should take a shower and avoid strenuous exercise and fatigue. A balanced diet is needed for patients who should eat more vegetables and high protein foods.
Pay attention to weather changes and keep warm. Be happy and relax, try not to participate in social activities, keep away from crowded and air-pollution. Patients should contact your doctor in time if you have abnormal abdominal pain, bloating, and vaginal bleeding, etc.
Hormone supplementation after transfer
After embryo transfer, patients need progesterone to support the corpus luteum. If the corpus luteum is not fully functional, it will increase the risk of miscarriage.
Progesterone is conducive to embryo implantation and inhibits uterine contraction. Moreover, Progesterone plays an important role in protecting fetuses, and participates in embryo protective immune regulation, increases fetal insulin secretion and promotes fetal growth.
Generally, 14 days after embryo transfer, urine test or blood draw can be used to determine pregnancy. If the pregnancy is confirmed, the doctor will prescribe supplement medicines according to the patient's condition. It is usually supplemented to 10-12 weeks to ensure the normal development of the fetus.
The above seven steps you will definitely go through in the IVF process. Besides, if you want to have a high success rate of IVF, you also need good embryos, suitable endometrium, and a good mood. Please trust your doctor and complete this "great project" together with your doctor!